genetically modify wine yeast

genetically modify wine yeast Description Hypothesis: overexpression of GDP1 gene combined with deletion of ALD6 gene in wine yeast strain can decrease the yield of alcohol production throughout the winemaking process. Experimental design: by overexpressing GDP1 gene which is primarily responsible for encoding isozymes glycerol 3-phosphate dehydrogenase, the yield of glycerol production will increase significantly and enhanced acetic acid production which results in decreased ethanol yield. However, in order to reverse the negative impact of a high concentration of acetic acid, we will delete the ALD6 gene which encodes for aldehyde dehydrogenase.